Tiny Case Study: Breastmilk's Healing Properties

Smiling woman wearing a hat, joyfully holding a baby dressed in a white shirt and denim pants, with an outdoor background

Summary

Our gut health report gives you deep insights into your baby's gut health. See a sample
Our gut health report gives you deep insights into your baby's gut health. See a sample

Khloe was about to become a mother for the second time at the age of 35. She was health conscious, consumed a mostly organic diet, and rarely ate processed or refined foods. Her first pregnancy had gone smoothly, and now she was expecting another tiny member of the family.

Scatter plot showing the principal component analysis results of various sample types, with data points representing different Gut types.
At 7 days old, Daniel had a Baby Gut Type dominated by unfriendly bacteria Enterobacteriaceae.

A rough start

Khloe had the support she needed to start breastfeeding within the first hour of giving birth to Daniel and she was grateful to have the opportunity to exclusively breastfeed.

Throughout the process, we worked with Khloe and helped her track her baby’s gut microbiome development.

This includes:

  • Khloe’s stool and vaginal sample, to check what she might pass along
  • Daniel’s 7-day stool sample, to check which microbes colonized
  • Daniel’s 30-day stool sample, to look for any changes

While we found beneficial species (like Bifidobacterium infantis) in Khloe’s stool sample, seeding was off to a rough start.  

She had a somewhat long 15-hour active labor and pushing at the hospital with increased medical interventions, like an epidural, multiple vaginal exams, and artificial rupture of the membranes (AROM) by the doctor, which may have affected the type of bacteria that seeded her baby at birth. It’s also interesting to note that Khloe got sick a lot during pregnancy and had a really bad cold for two weeks in her 2nd trimester and took Tylenol for her fever.

By the time Daniel was 7 days old (which is usually the earliest baby stool sample we take), his microbiome was mostly dominated by a group of unfriendly microbes called Enterobacteriaceae, which is more commonly found in the hospital environment than in a healthy baby’s gut.  

Stacked bar chart showing the composition of gut microbiota in babies aged 7 to 15 days old.

You can see in Khloe’s seeding chart below, that despite a vaginal birth she only transferred 3.7% of her microbes to her baby, while the other 96.3% was from the environment. The environment could include microbes from the hospital, hospital equipment, and doctors or nurses who may have initially interfaced with the baby. We would have expected to see more beneficial seeding from mom to baby in a vaginal birth.

Pie chart illustrating the sources of gut microbiota in infants, with 96.3% attributed to the environment and 3.7% attributed to the mother


Four pie charts comparing the sources of gut microbiota in infants based on birth method

This may explain why Daniel had a mild form of eczema on his chest and arms along with gas issues in his first month.

Baby probiotics that didn’t work

Since Daniel had tummy troubles in his first month and was very fussy, Khloe decided to try a baby probiotic that she found at her local Whole Foods store and fed it to Daniel for 4 weeks.

But his symptoms didn’t subside at all, so she stopped after 4 weeks.

Through Tiny Health’s baby gut test, we were able to see that this probiotic did not have any strains that might colonize a baby’s gut and persist. When an infant’s gut has very high levels of unfriendly bacteria, a good baby probiotic contains strains that can colonize the baby’s gut, crowding potentially harmful bacteria.

Breastfeeding can help since breastmilk is not just food for the baby but also for the beneficial Bifidobacterium in the baby’s gut.

Image of the supplement facts label on a product box, detailing serving size, servings per container, and proprietary blend total cultures.
Note that at the time this probiotic was given to the baby, Colic Calm had this older probiotic formulation. They have since completely changed their formulation.
Table showing the colonization rates of different probiotic strains in the Colic Calm Probiotic Formulation at 30 days
The table above shows that the specific probiotic strains in the baby probiotic barely colonized Daniel’s gut. With the right probiotic, we would have expected this to make up at least 30-50% of the baby’s gut after 1 month of supplementation.

Tiny Health demonstrates breastmilk’s healing properties

Khloe had high levels of beneficial Bifidobacterium in her gut while pregnant, as seen in the graph below. Due to modern lifestyle or antibiotic use, we see these at pretty low levels in many pregnant moms that we work with. This study shows that 90% of infants in the US do not inherit the beneficial B. infantis from their moms at birth, which are one of the four bacteria essential for their lifelong health. So the fact that Khloe has really high levels of Bifidobacterium in general is a great thing for her own health.

Table showing the presence and percentage of beneficial Bifidobacterium species including: infantis, bifidum, longum, and breve


In this table above, we point out 4 species of Bifidobacterium that are essential for a baby’s gut health. While Khloe had lower levels of these species, she did have 3 out of 4 of them (go, Khloe!). If she breastfeeds, there is a high chance that one of these species will colonize and dominate her baby’s gut, which is what we want to see in the first year. 

Bar chart comparing gut microbiota compositions: Khloe (39 weeks pregnant), Daniel (7 days old), and Daniel (30 days old)

By the time Daniel was 30-days old, his gut microbiome had transitioned to a healthy Bifidobacterium-dominated community. And we can see through his gut test results that it wasn’t the probiotic for colic that Khloe had used early on. Instead, it was the healing properties of his mother’s breastmilk!  

How could this be?

Table showing the association of specific Bifidobacterium species with baby’s immune health, diaper rash, colic, and fussiness across 3 time points

Thanks to breastmilk, and the special sugars found in breastmilk known as HMOs (human milk oligosaccharides) that feed Bifidobacterium, the beneficial bacteria flourished and pushed out the unfriendly bacteria from 73.5% at birth to 17.6% at 30-days, which is friendly to Daniel’s overall immune development. Breastmilk also produces special compounds, like lactate and acetate, that discourage the growth of unfriendly bacteria.

Seeing Daniel’s Tiny Health results encouraged Khloe to continue exclusively breastfeeding for as long as possible. She ended up breastfeeding for 12 months.

If you’re wondering whether the conditions during your baby’s birth influenced their microbiome seeding, whether a specific probiotic you’re giving worked, or just to simply witness the magic of your breastfeeding efforts, consider Tiny Health’s microbiome tests for pregnancy and for baby.

A screenshot of the Tiny Health web application showing the gut test results

Take a look inside our baby gut health report

Plus, get tips for baby gut health straight to your inbox from our team of experts